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KMID : 0380619820140010072
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Korean Journal of Food Science and Technology
1982 Volume.14 No. 1 p.72 ~ p.79
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Studies on the Mutation of Aspergillus niger
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Abstract
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Several mutants were isolated from the parent strain of Aspergillus niger CF: the first mutant strain CF-11 was obtained by UV irradiation, and the second mutant strain CF-11 and CF-22 were from NTG (N-methyl-N´-nitroso-N-nitroso-guanidine) treatment on the CF-11. These mutants were characterized, and their enzyme and acid production on wheat bran Koji and wheat flour Koji were studied.
Asp. niger CF-22 mutant appeared to be tan type which conidial heads were discolored. It´s glucoamylase activity was inreased approximately two times and its α-amylase about 50 percent as compared with that of the parent strain of Asp. niger CF, when grown on wheat bran Koji under the optimal conditions.
Asp. niger CF-21 mutant showed slower growth rate and poor sporulation than the wild type, although its conidial heads were not discolored. Approximately 4-fold increment in its acid production was observed as compared with the weld type.
The activities of glucoamylase and α-amylase of the Asp. niger CF-22 and CF-21 mutants were higher than those of the wild type, but their protease activity was rather lower.
The maximum production of glucoamylase by the Asp. niger CF-22 mutant was obtained after 2 to 3 days incubation on wheat bran at 30 to 30℃; α-amylase 2 days incubation at 30 to 35℃.
The maximal levels of acid production by the mutant CF-21 was appeared after 2 days incubation on wheat bran Koji, and after 3 days on wheat flour Koji at 30℃. Little differences in the levels of acid production were observed between on wheat bran and flour Koji.
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